Planta Med 2008; 74 - P-123
DOI: 10.1055/s-2008-1075319

Site Specific Comparision of Secondary Metabolites of Stylissa massa by Hplc Chemical Fingerprinting

S Ankisetty 1, M Slattery 1, 2
  • 1Department of Pharmacognosy and
  • 2National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS 38677-1848

Bromopyrrole alkaloids exhibiting a variety of biological activities are characteristic secondary metabolites found in sponges belonging to the genus Stylissa. The crude 1:1 DCM:MeOH extract of the sponge exhibited activity against C. neoformans with an IC50 of 0.2 µg/ml. Bioassay guided fractionation resulted in the isolation of the active component palau'amine, a hexacyclic bisguanidine alkaloid which was first isolated from Stylotella agminata, currently under study for its antifungal, antitumor and imuunosuppresive activities and currently undergoing preclinical studies. We were interested to see how Palau'amine content varies in sponges collected from different sites. Several other known compounds hymenidin, oroidin, sceptrin, hymenialdisine, dibromophakellin, 2,3-dibromoisopalauamine, 4,5-dibromopalauamine were also isolated from Stylissa massa collected in American Samoa. The isolated compounds were used as marker compounds and the crude extracts from Guam, American Samoa, Saipan and Pohnpei were compared by HPLC fingerprinting. The results of HPLC analysis will be presented.