The Ability of Wild Carrot Cell Cultures to Retain Their Capacity for Anthocyanin Synthesis After Storage at -140° C

Abstract

Twenty-five different anthocyanin producing cultures of wild carrot were stored at -140° and recovered. In all cases, viable cultures were recovered which produced approximately the same amount of anthocyanin as did similar cultures which had not been frozen and thawed. This is the first demonstration that plant cell cultures retain their ability to produce specific chemicals after cryogenic storage. In addition, the data provided show that a reciprocal relationship between growth of cultures and their ability to produce specific chemicals does not always hold true.

Key Word Index

Cell Cultures - Secondary Products - Cryogenic Storage.