Abstract
Callus cultures of Japanese plumyew, Cephalotaxus harringtonia, on Mura-shige and Skoog medium grew best at 25° C. Initial pH was unimportant in the
range of 4.5 to 8.0, and final pH was always 4.2. The tissues after six transfers
grew well on a vitamin-free medium of salts, sucrose and hormones. Varying the levels
of NH4NO3, protein hydrolysate, micronutrients (Mn, Zn, Mo, Cu, Co, I, B), sucrose and kinetin
in a 25 factorial experiment showed that response to the variation of one component depended
on the levels of the others. For example, adding NH4-NO3 increased growth 69 °/o if all other components were at optimal levels, yet decreased
growth 58 % if they were suboptimal.
Key Word Index
Cephalotaxus harringtonia - Tissue Culture - Growth - Culture Conditions - Statistical
Interactions.
