Planta Med 1980; 40(10): 218-223
DOI: 10.1055/s-2008-1074962
Research Articles

© Georg Thieme Verlag Stuttgart · New York

Biotransformation of Cardiac Glycosides by Immobilized Cells of Digitalis lanata1

A. Wilhelm Alfermann, Irmela Schuller, Ernst Reinhard
  • Lehrstuhl für Pharmazeutische Biologie, Universität Tübingen, Federal Republic of Germany
1 Dedicated to Prof. Dr. Drs. h.c. KURT MOTHES on the occasion of his 80th birthday
Further Information

Publication History

Publication Date:
29 April 2008 (online)

Abstract

Biotransformation of digitoxin and of ß-methyldigitoxin by Digitalis lanata cells entrapped in alginate gel was investigated. In shake cultures of both freely suspended and of entrapped cells, respectively, the biotransformation products of each substrate are quite the same. Most of the added digitoxin is glucosylated to purpureaglycoside A, whereas digoxin and deacetyllanatoside C are found only in minute amounts. ß-Methyldigitoxin is hydroxylated to ß-methyldigoxin with only small amounts of the side product purpureaglycoside A. The hydroxylating activity of the entrapped cells is about one half of that of freely suspended cells under the same cultural conditions. Advantageously, the immobilized biocatalyst can be used for more than 60 days.