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Planta Med 1980; 40(10): 92-103
DOI: 10.1055/s-2008-1074945
Research Articles

© Georg Thieme Verlag Stuttgart · New York

Growth, Morphogenesis and Cardenolide Formation in Long-term Cultures of Digitalis lanata1

R. Garve1 , M. Luckner1 , E. Vogel1 , A. Tewes2 , L. Nover2
  • 1Section of Pharmacy of the Martin-Luther-University, DDR-402 Halle, Weinbergweg 15
  • 2Institute of Plant Biochemistry of the Academy of Sciences of GDR, DDR-402 Halle, Weinberg 3
1 Dedicated to Prof. Dr. Drs. h. c. K. MOTHES on occasion of his 80th birthday
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Publikationsdatum:
29. April 2008 (online)

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Abstract

The establishment of two different types of long-term cultures of Digitalis lanata is described. Characteristics of their development and conditions for triggering organogenetic processes are discussed. Radioimmunological analyses of these culteres revealed a) that a low level of cardenolides (about 1 ng/mg dry wt.) is always present and b) that in light-grown cultures a 10 - 100 fold increase is observed in media with low auxin activity which favours the formation of green globular structures and embryoids. Under suitable condition in suspension cultures cardenohde content reaches 1 mg/l culture within 20 days. The cardenolides formed are mainly digitoxigenin derivatives that are mostly retained in the cell material.

Key Word Index

Digitalis lanata - Scrophulariaceae - Cardenolides - Digoxin - Digitoxin - Radioimmunoassay - Cell Cultures - Morphogenesis - Secondary Metabolism.