Planta Medica, Inhaltsverzeichnis Planta Med 1980; 39(8): 375-382DOI: 10.1055/s-2008-1074932 Research Articles © Georg Thieme Verlag Stuttgart · New YorkProtoplast-derived Clones from Cell Cultures of Digitalis purpureaB. Diettrich1 , D. Neumann2 , M. Luckner1 1Martin-Luther-University, Section of Pharmacy, Halle, German Democratic Republic 2Institute of Plant Biochemistry, Academy of Sciences of the German Democratic Republic, Halle Artikel empfehlen Abstract als PDF herunterladen Artikel einzeln kaufen Abstract Protoplasts were obtained with high yield from cells of suspension cultures of Digitalis purpurea by incubation with a mixture of cellulase R 10 Onozuka and macerozyme RIO. They were cultivated in a solidified, conditioned Murashige-Skoog medium with 30 μM kinetin and 0.3 M mannitol. Within 24 hours the protoplasts regenerated walls. These single cells underwent subsequent divisions forming clusters of 30-40 cells within 4 weeks. The protoplast-derived cells, as well as the cells of the suspension cultures used for protoplast preparation, obviously divide by means of a centripetal mechanism, deviating from the centrifugal one typical for cells of higher plants. Key Word Index Cell Culture - Protoplasts - Cell Wall Formation - donation of Cells - Digitalis purpurea. PDF (932 kb)
