Alkaloid Pattern of Cell Suspension Cultures and Differentiated Plants of Lupinus polyphyllus

Abstract

Using glass capillary gas-liquid chromatography and mass spectrometry the alkaloid patterns of Lupinus polyphyllus LiNDL. plants and cell suspension cultures were analyzed. Sparteine, angustifoline, lupanine, 13-hydroxylupanine, 17-oxolupanine, 13-cinnamoyllupanine (new for L. polyphyllus), 13-tigloyllupanine and its cis-isomer 13-angeloyllupanine (as a new alkaloid), and a dehydrolupanine were found in L. polyphyllus leaves and seeds. In addition the presence of other new quinolizidine alkaloids could be established, too. Cell suspension cultures produce lupanine as the main alkaloid. The total alkaloid concentration of cell cultures is more than 200 times lower than that of differentiated lupins, although their biosynthetic capacity to produce alkaloids is actively expressed as has been shown by precursor feeding experiments and enzymatic studies.

Key Word Index

Lupinus polyphyllus - Fabaceae (Leguminosae) - Quinolizidine Alkaloids - Cell Suspension Culture - Capillary GC-MS.