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DOI: 10.1055/s-2008-1062711
© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York
Role of Hyperglycemia in Isogeneic Islet Transplantation: An Experimental Animal Study
Publication History
received 24.01.2008
first decision 18.02.2008
accepted 18.02.2008
Publication Date:
13 May 2008 (online)
Abstract
Objective: Study the role of hyperglycemia-induced β cell loss on grafted islet destruction.
Design: Male inbred rats were made diabetic by streptozotocin administration and used as islet donors and/or isograft recipients to probe directly the role of hyperglycemia as an important determinant of transplanted islet fate, following exclusion of immune-related causes of islet graft destruction like allograft immunity and disease recurrence.
Results: Our studies showed that: a) Hyperglycemia destroyed islet but not pituitary isografts and b) Tight control of normoglycemia by sufficient islet mass engraftment prevented graft damage.
Conclusion: While sustained hyperglycemia caused destruction of transplanted islet isografts, induction of normoglycemia by transplantation of sufficient islet mass to diabetic recipients had a beneficial long term effect on their functional engraftment.
Key words
glucose metabolism - pancreas - insulin - islet transplantation
References
- 1 Ar’ Rajab Ahren B. Prevention of hyperglycemia improves the long term results of islet transplantation in streptozotocin – diabetic rats. Pancreas. 1992; 7 435-442
- 2 Babcock SK, Gill RG, Bellgrau D, Lafferty KJ. Studies on the two signal model for T cell activation in vivo. Transpl Proc. 1987; 19 ((1)) 303-306
- 3 Biarnes M, Montolio M, Nacher V, Raurell M, Soler J, Montanya E. Beta-cell death and mass in syngeneically transplanted islets exposed to short- and long-term hyperglycemia. Diabetes. 2002; 51 66-72
- 4 Emamaullee JA, Stanton L, Schur C, Shapiro AM. Caspase inhibitor therapy enhances marginal mass islet graft survival and preserves long-term function in islet transplantation. Diabetes. 2007; 56 1289-1298
- 5 Ferrer-Garcia JC, Merino-Torres JF, Perez Bermejo G, Herrera-Vela C, Ponce-Marco JL, Pinon-Selles F. Insulin induced normoglycemia reduces islet number needed to achieve normoglycemia after allogeneic islet transplantation in diabetic mice. Cell Transplant.. 2003; 12 849-857
- 6 Gray DWR, Cranston D, MacShane P, Sutton R, Morris PJ. Preferential effect of hyperglycemia on pancreatic islets transplanted into rats beneath the kidney capsule. Diabetologia. 1989; 32 663-667
- 7 Inoguchi T, Battan R, Handler E, Sportsman JR, Heath M, King GL. Preferential elevation of protein kinase C isoform beta II and diacylglycerol levels in the aorta and heart of diabetic rats: differential reversibility to glycemic control by islet transplantation. Proc Natl Acad Sci. 1992; 89 1059-1063
-
8 Lafferty KJ, Gill RL, Babcock SK, Simeonovic CJ. Activation and expression of allograft immunity.
In: Progress in Transplantation . 1986: 55-84 - 9 Lafferty KJ, Prowse SJ, Simeonovic CJ. Immunobiology of tissue transplantation: a return to the passenger leucocyte concept. Annu Rev Immunol. 1983; 1 143-173
- 10 Merino JF, Nacher V, Raurell M, Biarnes M, Soler J, Montanya E. Optimal insulin treatment in syngeneic islet transplantation. Cell Transplant. 2000; 9 11-18
- 11 Meyer C. Islet transplantation. N Engl J Med. 2007 Mar 1; 356 ((9)) 963 , ; author reply 964–965
- 12 Morsiani E, Fogli L, Lanza Jr G, Lebow LT, Demetriou AA, Rozga J. Long-term insulin independence following repeated islet transplantation in totally pancreatectomized diabetic pigs. Cell Transplant. 2002; 11 55-66
- 13 Nomikos IN, Malizos C, Vamvakopoulos NC. Protective and damaging effects of healing. Wounds. 2006; 18 177-185
- 14 Nomikos IN, Prowse SJ, Carotenuto P, lafferty KJ. Combined treatment with nicotinamide and desferrioxamine prevents islet allograft destruction. Diabetes. 1986; 35 1302-1304
- 15 O’Connell PJ, Hawthorne WJ, Holmes-Walker DJ, Nankivell BJ, Gunton JE, Patel AT, Walters SN, Pleass HC, Allen RD, Chapman JR. Clinical islet transplantation in type 1 diabetes mellitus: results of Australia's first trial. Med J Aust. 2006; 184 ((5)) 221-225
- 16 Prowse SJ, Bellgraau D, Lafferty KJ. Islet allografts are destroyed by disease recurrence in spontaneous diabetic BB rat. Diabetes. 1986; 35 110-114
- 17 Rajotte RV, Warnock GL, Kneteman NM. Cryopreservation of insulin-producing tissue in rats and dogs. World J Surg. 1984; 8 179-186
- 18 Ryan EA, Shandro T, Green K, Paty BW, Senior PA, Bigam D, Shapiro AM, Vantyghem MC. Assessment of the severity of hypoglycemia and glycemic lability in type 1 diabetic subjects undergoing islet transplantation. Diabetes. 2004; (4) 955-962
- 19 Sioutopoulou D, Plakokefalos E, Anifandis G, Arvanitis L, Venizelos I, Valery R, Destouni H, Vamvakopoulos NC. Comparing normal primary endocervical adenoepithelial cells to uninfected and influenza B virus infected human cervical adenocarcinoma HeLa cells. Int J Gynecol Cancer. 2006; 16 2032-2038
- 20 Stafani MR, Dunlap JA, Yorek MA. Effect of L-fucose on proliferation and myo-inositol metabolismin cultured cerebral microvessel and aortic endothelial cells. J Cell Physiol. 1992; 153 321-331
- 21 Unger RH, Grundy S. Hyperglycemia as an inducer as well as a consequence of impaired islet cell function and insulin resistance: implications for the management of diabetes. Diabetologia. 1985; 28 118-121
- 22 Vakalis NC, Spanacos G, Patsoula Å, Vamvakopoulos NC. Improved detection of Dirofilaria repens DÍÁ by direct polymerase chain reaction. Parasitology International. 1999; 48 145-150
Correspondence
Dr. M. SatraPhD
Department of Biology
University of Thessaly
Papakuriazi -22
41 221 Larissa
Greece
Phone: +30/56/50 74
Email: msatra@med.uth.gr