Changes of Mental Stress Biomarkers in Ultramarathon

Abstract

We investigated the possible influence of an exhaustive physical exercise on mental stress biomarkers (serotonin, tryptophan, and β-endorphin) along with dopamine, noradrenalin and free fatty acids in an ultramarathon race in which 45 km was run on the first day and 90 km on the second. We obtained serum samples at 6 different time points during and after the race from 18 Japanese male runners who completed the marathon. Overall changes of serum serotonin and tryptophan concentrations were statistically significant according to ANOVA for repeated measurements (p < 0.05). Serum serotonin levels elevated rapidly on the first day with the post hoc Tukey's test. Tryptophan concentrations inversely decreased during the race, possibly because of utilization for synthesis of serotonin. Levels of β-endorphin appeared to increase on the first and second days, but were not statistically significant. In conclusion, serum serotonin, tryptophan and β-endorphin appeared to be used for mental stress markers in physical exercise.

Key words

ultramarathon - serotonin - tryptophan - β‐endorphin

References

• 1 Campbell P N, Smith A D. Biochemistry Illustrated. London; Churchill Livingstone 1988: 156
• 2 Fernstrom D J, Fernstrom H M. Exercise, serum free tryptophan, and central fatigue.  J Nutr. 2006;  136 553S-559S
• 3 Fournier P E, Stalder J, Mermillod B, Chantraine A. Effects of a 110 kilometers ultramarathon race on plasma hormone levels.  Int J Sports Med. 1997;  18 252-256
• 4 Goldfarb A H, Jamurtas A Z. Beta-endorphin response to exercise. An update.  Sports Med. 1997;  24 8-16
• 5 Jacobs B L. Brain monoaminergic unit activity in behaving animals.  Prog Psychobiol Physiol Psychol. 1987;  12 171-206
• 6 McBride W J, Murphy J M, Ikemoto S. Localization of brain reinforcement mechanisms: intracranial self-administration and intracranial place-conditioning studies.  Behav Brain Res. 1999;  101 129-152
• 7 Morgan W P. Affective beneficence of vigorous physical activity.  Med Sci Sports Exerc. 1985;  17 94-100
• 8 Odagiri Y, Shimomitsu T, Iwane H, Katsumura T. Relationship between exhaustive mood state and changes in stress hormones following an ultraendurance race.  Int J Sports Med. 1996;  17 325-331
• 9 Ohta M, Hirai N, Ono Y, Ohara M, Saito S, Horiguchi S, Watanabe M, Tokashiki A, Kawai A, Andou T, Shioji I, Noguchi T, Morizuka M, Suzuki M, Imanishi A, Takeda N, Machida K. Clinical biochemical evaluation of central fatigue with 24-hour continuous exercise.  Rinsho Byori. 2005;  53 802-809
• 10 Ohtsuki S. The physiological function of the blood-brain barrier transporters as the CNS supporting and protecting system.  Yakugaku Zasshi. 2004;  124 791-802
• 11 Petraglia F, Bacchi M A, Comitini G, Scazzina D, Facchinetti F, Fiaschetti D, Genazzani A D, Barletta C, Scavo D, Genazzani A R. Plasma beta-endorphin and beta-lipotropin levels increase in well trained athletes after competition and non competitive exercise.  J Endocrinol Invest. 1990;  13 19-23
• 12 Pietraszek M H, Takada Y, Yan D, Urano T, Serizawa K, Takada A. Relationship between serotonergic measures in periphery and the brain of mouse.  Life Sci. 1992;  51 75-82
• 13 Sano S, Kaba M, Sakamoto M, Sato-Suzuki I, Arita H. Activation of serotonergic system by step exercise in humans.  Hokuriku Daigaku Kiyo. 2002;  26 39-48
• 14 Struder H K, Weicker H. Physiology and pathophysiology of the serotonergic system and its implications on mental and physical performance. Part II.  Int J Sports Med. 2001;  22 482-497
• 15 Tatsumi N. Handbook of Reference Values of Clinical Tests. 2nd edn. Tokyo; Nankodo 2003 70 110 203
• 16 Tokudome S, Kuriki K, Yamada N, Ichikawa H, Miyata M, Shibata K, Hoshino H, Tsuge S, Tokudome M, Goto C, Tokudome Y, Kobayashi M, Goto H, Suzuki S, Okamoto Y, Ikeda M, Sato Y. Anthropometric, lifestyle and biomarker assessment of Japanese non-professional ultra-marathon runners.  J Epidemiol. 2004;  14 161-167
• 17 Vale W. Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and endorphin.  Science. 1981;  213 1394-1397