Planta Med 2008; 74(2): 197-200
DOI: 10.1055/s-2008-1034289
Biochemistry and Molecular Biology
Letter
© Georg Thieme Verlag KG Stuttgart · New York

Identification of Medicinal Ganoderma Species Based on PCR with Specific Primers and PCR-RFLP

Zhou Xuanwei1 , Li Qizhang1 , Yin Yizhou2 , Chen Yiyuan3 , Lin Juan2
  • 1Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, P. R. China
  • 2State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai, P. R. China
  • 3College of Life Sciences, Sichuan University, Chengdu, P. R. China
Further Information

Publication History

Received: August 19, 2007 Revised: November 29, 2007

Accepted: December 14, 2007

Publication Date:
04 February 2008 (online)

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Abstract

Ganoderma lucidum is an interesting shelf fungus that is also of medical importance in East Asian countries such as China, Japan and Korea. The fruit bodies of different Ganoderma species have similar colors and shapes, but are different regarding their medicinal properties. In order to provide molecular evidence for the genuine origin of G. lucidum, G. sinense and G. tsuga, fungal immunomodulatory protein (FIP) genes were cloned using genomic walker technology and their sequences were then determined. Based on the sequences of cloned FIP genes, we designed three pairs of species-specific primers for amplification and sequencing of FIP genes. These new primers allowed an effective amplification and sequencing of FIP genes from representative species of G. lucidum, G. sinense and G. tsuga. Phylogenetic relationships were resolved quite efficiently by the FIP gene sequences, which proved to be more useful than other genetic marker techniques in phylogenetic reconstruction of G. lucidum, G. sinense and G. tsuga.

References

Prof. Xuanwei Zhou

Plant Biotechnology Research Center

Fudan-SJTU-Nottingham Plant Biotechnology R&D Center

School of Agriculture and Biology

Shanghai Jiao Tong University

Huashan Road 1954

Shanghai 200030

People’s Republic of China

Phone: +86-21-6564-2425

Fax: +86-21-6564-2425

Email: xuanweizhou@sjtu.edu.cn

Prof. Juan Lin, Ph.D.

State Key Laboratory of Genetic Engineering

School of Life Sciences

Morgan-Tan International Center for Life Sciences

Fudan-SJTU-Nottingham Plant Biotechnology R&D Center

Fudan University

220 Handan Road

Shanghai 200433

People’s Republic of China

Phone: +86-21-6564-2772

Fax: +86-21-6564-3552

Email: linjuan@fudan.edu.cn