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DOI: 10.1055/s-2008-1034277
© Georg Thieme Verlag KG Stuttgart · New York
A Phytochemically Characterized Extract of Cordyceps militaris and Cordycepin Protect Hippocampal Neurons from Ischemic Injury in Gerbils
Publikationsverlauf
Received: April 12, 2007
Revised: April 12, 2007
Accepted: December 3, 2007
Publikationsdatum:
23. Januar 2008 (online)
Abstract
In the present study, we investigated effects of the dried, hot-water extract of Cordyceps militaris (CME) and its major metabolite (cordycepin) against ischemic damage. The repeated treatment with CME protected hippocampal CA1 pyramidal neurons from ischemic damage in gerbils. The treatment with CME or cordycepin in gerbils reduced 4-hydroxy-2-nonenal (a marker of lipid peroxidation) immunoreactivity and levels in the ischemic CA1 region. Glial fibrillary acidic protein immunoreactive astrocytes and ionized calcium-binding adapter molecule 1 immunoreactive microglia in the vehicle-treated ischemic group were activated in the CA1 region 4 days after ischemia/reperfusion, whereas in the CME- or cordycepin-treated ischemic group, their activation was significantly decreased. These results suggest that the repeated treatment with CME protects against neuronal damage from ischemia/reperfusion by reducing oxidative damage.
Abbreviations
CME:Cordyceps militaris water extract
CV:cresyl violet
GFAP:glial fibrillary acidic protein
HNE:4-hydroxy-2-nonenal
HPLC:high performance liquid chromatography
Iba-1:ionized calcium-binding adapter molecule 1
ROD:relative optical density
ROS:reactive oxygen species
Key words
Neuroprotection - ischemia - pyramidal neurons - hippocampus - Cordyceps militaris - cordycepin - fungus
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1 In Koo Hwang and Soon Sung Lim contributed equally to this article
Prof. Moo-Ho Won
Department of Anatomy and Neurobiology
College of Medicine
Hallym University
Chuncheon 200-702
South Korea
Telefon: +82-33-248-2522
Fax: +82-33-256-1614
eMail: mhwon@hallym.ac.kr