Ipodate and 8-Anilino-1-Naphthalene Sulfonic Acid Block Receptor Binding of T₃ in Rat Liver

Summary

We have determined that 8-anilino-l-naphthalene sulfonic acid (ANS) and ipodate are effective inhibitor in vitro of ¹²⁵I-T₃ binding to rat hepatic nuclei receptors. Both of these agents are estimated to have a K_d for the T₃ receptor of about 1-2 × 10^-4 M. Indirect preliminary studies suggest that ANS is a non-competitive inhibitor and ipodate is a competitive inhibitor of T₃ binding. Compounds such as tyropanoate and diatrizoate and iodide had no effect on T₃ receptor binding. Further in vivo studies with ipodate suggested that T₃ receptor binding inhibition also occurred when ipodate was given intravenously to rats.

T ₃ Receptor - 8-Anilino-l-Naphthalene Sulfonic Acid - Ipodate