Subscribe to RSS
DOI: 10.1055/s-2007-999190
Effect of Glucoprivation Induced by 2-deoxy-D-Glucose on Serum Gonadotropin Levels, Pituitary Response to GnRH and Progesterone-Induced Release of Luteinizing Hormone in Rats
Publication History
1979
1980
Publication Date:
14 March 2008 (online)
Summary
Intact (experiment 1) or castrated (experiment 2) male rats were either fed or fasted for 24 hours. Half of each group received 75 mg 2-deoxy-D-glucose/100 gm body weight, intraperitoneally. Assays of sera from blood samples obtained from intact rats 4 hr after the injection revealed that 2-de-oxy-D-glucose suppressed serum testosterone levels (P < 0.05) while 2-deoxy-D-glucose plus fasting was most effective in suppressing LH and FSH. In castrates LH was suppressed by 2-deoxy-D-glucose and FSH by fasting (P < 0.01 and P < 0.05). Serum glucose was elevated 3-5 fold by 2-deoxy-D-glucose (P < 0.01).
Experiments with female rats revealed that treatment with 2-deoxy-D-glucose had no effect on the rise in serum LH that followed GnRH treatment in ovariectomized, steroid-primed animals. However, progesterone-induced LH release in estrogen-primed ovariectomized rats was blocked by 2-deoxy-D-glucose. Collectively, these results suggest that glucoprivation impairs hypothalamic control of gonadotropin secretion.
Key-Words
2-Deoxy-D-Glucose - Fasting - Luteinizing Hormone - Follicle Stimulating Hormone