Summary
The ability of adipocytes from lipomas to respond to lipolytic stimuli was investigated
to determine whether diminished responsiveness could account for the excessive accumulation
of neutral lipid which characterizes these tumors.
Baseline studies were performed on rat adipose tissues to determine optimal conditions
for comparison of lipolytic responses to a variety of hormones. Cell concentrations,
the specific depot site from which the cells are derived, the age of the animal and
the tissue preparation, as to whether fragments are used as opposed to collagenase-treated
isolated cells, were determined to affect the lipolytic response. When the response
of lipomata adjusted per unit cell (DNA) are compared to unaffected cells adjoining
the tumor, an enhanced lipolytic response to μM concentrations of l-epinephrine is
seen in both fragments and isolated cells. These findings indicate that diminished
lipolysis cannot account for the exaggerated storage of neutral fat in these tumors.
Key-Words
Lipoma
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Isolated Fat Cells
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L-Epinephrine
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ACTH
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TSH
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Glucagon
