Abstract
The electron microscope is generally regarded as a sophisticated instrument used almost
exclusively for basic research. However, ultrastructural methods can be just as valuable
for the clinical diagnosis of inherited platelet disorders, as for more fundamental
studies. This report describes several instances in which electron microscopy has
been critical for identifying and characterizing genetic problems. For example, platelet
storage pool deficiency (SPD) is due to a marked decrease or absence of the organelles
storing adenine nucleotides, serotonin, and calcium destined for secretion during
the platelet release reaction. The organelles are referred to as dense bodies because
they are inherently electron opaque. As a result, platelet SPD is more rapidly and
reliably diagnosed in the electron microscope than by any other technique. Giant platelet
disorders have presented a bewildering array. The electron microscope has made it
possible to separate the various types into distinct conditions based on the nature
of platelet structural defects and inclusions found in leukocytes. Immunogold and
cytochemical techniques have expanded the horizon of electron microscopy in the evaluation
of platelet disorders and have assured its continued use for this purpose in the future.
Keywords:
Platelets - electron microscopy - storage pool deficiency - giant platelet disorders
- thrombocytopathies
