Abstract
Herba Oldenlandiae is the dried whole herb of Oldenlandia diffusa (Willd.) Roxb. (Family Rubiaceae) recorded in the Chinese pharmacopoeia. However,
the herbs of O. corymbosa (L.) Lam and O. tenelliflora Bl. are also often available and used indiscriminately as Herba Oldenlandiae in the
markets. In light of this, high-performance liquid chromatography (HPLC) combined
with high resolution mass spectrometry (HR-MS) has been developed for a comparative
analysis of chemical components in the three Oldenlandia species. The correlation coefficients of the entire chromatographic patterns of samples
were calculated by software and used for appraising the similarity. The chemical information
on the peaks in the chromatograms of O. diffusa was obtained by comparison of their exact mass data, UV spectra and literature values.
The results indicated that the chemical profiling of O. diffusa is quite different in samples collected from different habitats. Furthermore, the
variations in the abundance of asperuloside, E-6-O-p-coumaroylscandoside methyl ester and E-6-O-p-coumaroylscandoside methyl ester-10-methyl ether were significant in these related
herbs. In conclusion, it is important to use the above three compounds as chemical
markers for quality evaluation and chemical authentication of Herba Oldenlandiae and
its substitutes.
Key words
Rubiaceae -
Oldenlandia diffusa
- substitute - fingerprint - high-performance liquid chromatography
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Prof. Dr. Zhongzhen Zhao
School of Chinese Medicine
Hong Kong Baptist University
Kowloon
Hong Kong Special Administrative Region
People’s Republic of China
Phone: +852-3411-2424
Fax: +852-3411-2461
Email: zzzhao@hkbu.edu.hk
