Planta Med 2007; 73(14): 1452-1456
DOI: 10.1055/s-2007-990255
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Cytotoxic Activities of Xanthochymol and Isoxanthochymol Substantiated by LC-MS/MS[*]

Satyanshu Kumar1 , Sunil K. Chattopadhyay1 , Mahendra P. Darokar1 , Ankur Garg1 , Suman P. S. Khanuja1
  • 1Central Institute of Medicinal and Aromatic Plants (CIMAP), P.O. CIMAP, Lucknow, India
Further Information

Publication History

Received: June 18, 2007 Revised: September 21, 2007

Accepted: September 24, 2007

Publication Date:
12 November 2007 (online)

Abstract

The methanol extract of the fruit rinds of Garcinia indica showed potent cytotoxic activity against three human cancer cell lines, colon (COLO-320-DM), breast (MCF-7) and liver (WRL-68), as determined by the MTT assay. Fractionation of the methanol extract into hexane-, chloroform- and ethyl acetate-soluble fractions and evaluation of cytotoxic activity of each of the fractions revealed that the ethyl acetate fraction was the most effective as compared to the two other fractions. Two polyisoprenylated benzophenones, xanthochymol and isoxanthochymol, were isolated from the ethyl acetate fraction. Both xanthochymol and isoxanthochymol as a single pure entity did not turn out to be as effective as the ethyl acetate extract from which they have been isolated. The concentrations of xanthochymol and isoxanthochymol in four different extracts - methanol, hexane, chloroform and ethyl acetate - were determined with the help of LC-MS/MS. On the basis of the LC-MS/MS data, combinations of xanthochymol and isoxanthochymol in different ratios were tested in the MTT assay and were found to be effective. Moreover, it was established from the LC-MS/MS data that the combination of xanthochymol and isoxanthochymol in a ratio of 1 : 2 showed maximal cytotoxicity

1 CIMAP Communication No. 2007-53J

References

1 CIMAP Communication No. 2007-53J

Sunil K. Chattopadhyay

Central Institute of Medicinal and Aromatic Plants (CIMAP)

P.O. CIMAP

Lucknow - 226015

India

Phone: +91-522-235-9628

Fax: +91-522-234-2666

Email: chattsk@yahoo.com

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