Free radical scavenging activity of mushrooms from order Polyporales

Free radical scavenging activity of crude ethanol extracts from the fruiting bodies of 32 taxons of Polyporales mushrooms was evaluated by means of DPPH (2,2'-diphenyl-1-picrylhydrazyl radical) test using SIA (PC-controlled Sequential Injection Analysis system) method developed in our laboratory [1]. The most active samples were tested by means of hydrogen peroxide-induced luminol chemiluminescence assay using SIA [2]. The DPPH radical scavenging activity of the samples was expressed as 50% effective concentration (EC₅₀): Fomitopsis pinicola 0.086mg/ml, Osmoporus odoratus 0.276mg/ml, Phaeolus schweinityii 0.297mg/ml, Ganoderma lipsiense 0.379mg/ml, Daedaleopsis confragnosa 0.42mg/ml, Albatrellus ovinus 0.435mg/ml and Oligoporus ptychogaster 0.823mg/ml, and this values were compared with known antioxidants: acidum ascorbicum 0,006mg/ml, and trolox 0,014mg/ml. The results of the second method was also expressed as EC₅₀: F. pinicola 0,124mg/ml, G. lipsiense 0,273mg/ml, O. odoratus 0,393mg/ml and P. schweinitzii 0,573mg/ml and this values were compared with known antioxidants: trolox 0,025mg/ml, rutin 0,190mg/ml and caffeic acid 0,008mg/ml. The results at this point are promising for further research.

Acknowledgement: This research was financially supported by Charles University grant 2007

References: [1] Polasek, M. et al. (2004) Anal Bioanal Chem 379: 754–758. [2] Cheng, Z. et al. (2003) Anal Bioanal Chem 375: 376–380