A simple, precise and reproducible ultraviolet spectrophotometric method for the assay of powdered leaves, extracts and formulations of Loranthus micranthus was developed and validated. The complexation of the flavonoids of L. micranthus with methanolic aluminum nitrate solution was employed for the assay [1]. The absorbance of the formed complex was determined spectrophotometrically at λmax of 300 nm. The precision, repeatability, linearity, optical characteristics and accuracy of the method were studied [2]. Beer's law was obeyed in the concentration range of 0.4 to 3.6mg%. The calibration curve had a regression coefficient of 0.9913. The limit of detection and the limit of quantitation of the proposed method were found to be 0.04 and 0.12mg% respectively. Percentage recoveries between 92 and 112% were obtained. The proposed method proved to be robust and therefore recommended for the assay of powdered leaves, extracts and formulations of Loranthus micranthus.
References: [1] Crozier, A., Jennifer B., et. al., (2000). Biological Research 33, 3. [2] European Medicine Evaluation Agency (2006). Validation of analytical procedures: definitions and methodology, ICH Harmonised Tripartite Guideline, EMEA, London, UK. p1–15.
