Topical anti-inflammatory effect of acetone rhizome/root extract of Potentilla malýana Borbas

Potentilla malýana is an endemic plant from Balkan Peninsula, and there are no data about usage of this Potentilla. Plant material was collected in autumn 2006. The Acetone extract of the rhizome and root of Potentilla malýana has been evaluated for anti-inflammatory potential using the mouse ear edema model [1]. Animals (groups N=6) had food and water ad libitum during the test. Albino mice (6–7 weeks of age, average body weight 25g) were supplied from Institute for Pharmacology, Faculty of Medicine in Sarajevo. The animals were housed in climate-controlled quarters (24±°C at 50% humidity) with a 12-h light/12-h dark cycle. Ear inflammation was induced by 3% Oleum crotonis acetone solution, in quantity of 10µL on both mouse ears. Application of extract was only once on left ear, two hours after starting the inflammation. The right ear was the control (without any additional treatment). The ear was observed for three days, and appearance changes were expressed in scores from 0 to 14 [2]. Before application to a mouse ear, the acetone extract was prepared, which had total 5% of phenolic compounds [3]. Hydrocortisone 1% ointment was used as control on second group of the animals (N=6). The pharmacological reaction was found with the acetone extract of the rhizome Potentilla malýana, whose pharmacological reaction was weakest to hydrocortisone ointment. The score for treated ear was 8±1, for untreated ear 12±2, and for hydrocortisone ointment 5±1. The acetone extract of the plant Potentilla malýana exhibited significant reduced inflammation in time for 30–50% in relation to control ear.

References: [1] Grujić Vasić J., Pilipović S., Zulić I., Mijanović M., Redžić S. (2003): Works; Academy of Sciences and Arts of Bosnia and Herzegovina; Sarajevo. Vol. XCII:p 13–23 [2] Hayes A.W. (1984) Principles and Methods of Toksikology. Raven press, New York.p.212 [3] Pilipović S., Šober M., Bosnić T., Marjanović A. (2006) Arh. Farm. 56: 900–901