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Capsaicin-sensitive afferentation in Helicobacter pylori positive gastritis before and after eradication
Aims: The capsaicin-sensitive afferent nerves (CSANs) have an important role in protecting the gastric mucosa. The participation of CSANs in the development of chronic gastritis caused by different factors (Helicobacter pylori, drugs, etc.,) was studied earlier by our team (Dömötör et al., Dig. Dis. Sci. 2007. 52:411–417). Aim of the study: to investigate the immunhistochemical distribution of capsaicin receptor (transient receptor potencial vanilloid 1=TRPV1), calcitonin gene related peptide (CGRP), substance P (SP) in H. pylori positive chronic gastritis before and after eradication therapy. Patients and method: The biopsies of 18 patients (6 men, 12 women, mean age: 56.4 years) who suffered from H. pylori positive chronic gastritis were collected during gastro-duodenoscopy before and after eradication. H. pylori positivity was detected by the Warthin-Storry silver impregnation technique and urea breath test. TRPV1, CGRP and SP were detected by labeled polymer immunhistological method (Lab Vision Corp. USA) using polyclonal rabbit antisera (TRPV1, CGRP), and rat monoclonal antibody (SP) (Abcam Ltd, UK). 20 patients with functional dyspepsia were enrolled as histologically healthy controls. Results: Positive cases of TRPV1 immundistribution were 35% (n=7) in controls, 89% (n=16) before and 72% (n=13) after eradication. Immunhistochemistry of CGRP was positive in 40% (n=8) of controls, and in 100% (n=18) of patients before and after eradication. The SP immundistribution was negative in 75% (n=17) of control persons, 94,5% (n=17) in patients before and 100% (n=18) after eradication. Conclusion: 1. The capsaicin receptor (CSANs) and the CGRP are involved in the development of H. pylori positive chronic gastritis. 2. The time period between the gastro-duodenoscopies (before and after H. pylori eradication) could not be enough to change the immunhistochemical distribution of TRPV1, CGRP and SP in the gastric mucosa. 3. Not only H. pylori infections, but also other factors may be involved in the development of these cases of chronic gastritis.