Abstract
Gallotannins are plant-derived, water-soluble polyphenols with wide-ranging biological
activities. Mast cell-mediated allergic inflammation is known to cause many diseases
such as asthma, sinusitis, and rheumatoid arthritis. Mast cells induce synthesis and
production of pro-inflammatory cytokines including tumor necrosis factor (TNF)-α,
interleukin (IL)-1β, and IL-6 with immune regulatory properties. Expression of inflammatory
cytokines is mainly regulated by a transcription factor, nuclear factor (NF)-κB. In
the present study, the effect of eight gallotannins on the level of pro-inflammatory
cytokines and NF-κB activation was investigated in human mast cell line (HMC-1). HMC-1
cells were sensitized by phorbol 12-myristate 13-acetate (PMA) and calcium ionophore
(A23187). Among the eight gallotannins from Euphorbia species, three gallotannins such as 1,2,3,4,6-penta-O-galloyl-β-D-glucose, 1,2,6-tri-O-galloyl-β-D-allopyanose, and 1,2,3,6-tetra-O-galloyl-β-D-allopyranose suppressed the gene expression and secretion of pro-inflammatory cytokines
in a dose-dependent manner. In addition, these three gallotannins blocked the activation
of NF-κB as indicated by an NF-κB-dependent gene reporter assay. We conclude that
these gallotannins may have potential for the treatment of inflammatory diseases through
the down-regulation of NF-κB-mediated activation of mast cells.
Abbreviations
HMC-1:human mast cell
IL:interleukin
NF-κB:nuclear factor-κB
PMA:phorbol 12-myristate 13-acetate
PMACI:PMA and calcium ionophore A23187
Key words
Allose gallates -
Euphorbia species - Euphorbiaceae - mast cells - pro-inflammatory cytokines - nuclear factor-κB
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1 These authors contributed equally to this work
Prof. Sang-Hyun Kim
Department of Pharmacology
School of Medicine
Kyungpook National University
Daegu 700-422
Republic of Korea
Telefon: +82-53-420-4838
Fax: +82-53-423-4838
eMail: shkim72@knu.ac.kr
