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© Georg Thieme Verlag Stuttgart · New York
Effect of Acute Exhausting Exercise on Cytokine Gene Expression in Men
09 March 2007 (online)
Because of recent interest in the effects of physical exercise on immunologic function, we decided to use state-of-the-art methods to evaluate cytokines in the peripheral blood leukocytes (PBLs) of 7 men before and after a maximal treadmill stress test. Change in cytokine gene expression was quantified from PBLs using a reverse transcriptase polymerase chain reaction assay (RT-PCR). In contrast to reports on serum levels or using in vitro testing, direct gene expression of TNF-α decreased after the stress test (p < 0.008). However, the 47 % decrease was relatively small and of questionable biological significance. Levels of IL - 1a, IL-Ib. IL-2, IL-4, IL-6, IL-10. and INF-γ did not change.
Cytokines - stress testing - PCR - exercise