Int J Sports Med 1996; 17(4): 299-302
DOI: 10.1055/s-2007-972850

© Georg Thieme Verlag Stuttgart · New York

Effect of Acute Exhausting Exercise on Cytokine Gene Expression in Men

B. H. Natelson1 , X. Zhou2 , J. E. Ottenweller1 , M. T. Bergen1 , S. A. Sisto1 , S. Drastal1 , W. N. Tapp1 , W. L. Gause2
  • 1Chronic Fatigue Syndrome Center and Department of Neurosciences, UMDNJ - New Jersey Medical School, Newark NJ 07013
  • 2Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda MD 20814, U.S.A.
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Publication History

Publication Date:
09 March 2007 (online)

Because of recent interest in the effects of physical exercise on immunologic function, we decided to use state-of-the-art methods to evaluate cytokines in the peripheral blood leukocytes (PBLs) of 7 men before and after a maximal treadmill stress test. Change in cytokine gene expression was quantified from PBLs using a reverse transcriptase polymerase chain reaction assay (RT-PCR). In contrast to reports on serum levels or using in vitro testing, direct gene expression of TNF-α decreased after the stress test (p < 0.008). However, the 47 % decrease was relatively small and of questionable biological significance. Levels of IL - 1a, IL-Ib. IL-2, IL-4, IL-6, IL-10. and INF-γ did not change.