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Planta Med 1981; 41(1): 90-95
DOI: 10.1055/s-2007-971682
Research Articles

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Effects of Age and Growth Regulators on Serially Propagated Digitalis lanata Leaf and Root Cultures

Joseph H. C. Lui, E. J. Staba
  • College of Pharmacy, University of Minnesota, Minneapolis, Minnesota 55455
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Publikationsdatum:
29. März 2007 (online)

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Abstract

The cardenolide thin-layer chromatographic (TLC) and gasliquid chromatographic (GLC) patterns from leaf and root culture extracts were similar to those obtained from the plant. Leaf cultures at 8 and 12 wk-old contained more primary cardiac glycosides (Lanatoside A, B, C), than 4 wk-old leaf cultures. As high as 44 mg% dry wt. of digoxin, as determined by RIA, was present in 12 wk-old leaf cultures. The concentration of digoxin increased in both leaf and root cultures when treated with either gibberellic acid (GA) or mefluidide, and leaf organ cultures elongated with GA treatment.

Key Word Index

Digitalis lanata - Scrophulariaceae - Organ Culture - Cardenolide - Digoxin - Plant Growth Regulators