Abstract
Valepotriates, mainly isovaltrate and valtrate, have been separated and quantitatively
estimated by reversed-phase HPLC in the leaves, flowers, stems and rhizomes of Valeriana kilimandascharica. The isovaltrate/valtrate concentration reaches a maximum of 5.89% in the leaves,
3.84% in the flowers, 3.17% in the stems and 5.15% in the rhizomes.
A µ Bondapak C18 column using MeOH-H2O mixtures as eluant is suitable for a baseline separation of isovaltrate, valtrate,
acevaltrate and baldrinal at UV 254 nm in 15 min and didrovaltrate and IVHD-valtrat
at UV 208 nm in 10 min. Relative standard deviation for quantitative determinations
is approximately 1.5% for valepotriate contents of 1%. This method is adaptable for
routine analysis of crude extracts.
Key Word Index
Valeriana - Valerianaceae - Valepotriates - HPLC
