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Planta Med 1982; 46(9): 15-18
DOI: 10.1055/s-2007-970009
Research Articles

© Hippokrates Verlag Stuttgart

The Effects of Plant Growth Regulators and Culture Conditions on the Growth and the Alkaloid Content of Callus Cultures of Cinchona pubescens

Th. Mulder-Krieger, R. Verpoorte, Y. P. de Graaf, M. van der Kreek, A. Baerheim-Svendsen
  • Department of Pharmacognosy, State University of Leiden, Gorlaeus Laboratories, Leiden, The Netherlands
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Publication History

1982

Publication Date:
29 March 2007 (online)

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Abstract

Studies were performed on the growth and the alkaloid content of stem callus cultures of Cinchona pubescens VAHL concerning the influence of plant growth regulators in the medium. After two months the increase of fresh weight of cultures grown in the dark was less than those incubated with illumination.

A number of quinoline and indole alkaloids could be detected by means of TLC. Media F (zeatin: 1 µM, indole-3-butyric acid: 1 µM) and H (zeatin: 1 µM, 2,4-dichlorophenoxy acetic acid: 1 µM) showed the best alkaloid production and tissue growth.

Key Word Index

Cinchona pubescens - Rubiaceae - Tissue Culture - Alkaloids - Tissue Growth - Plant Growth Regulators

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