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Planta Med 1983; 47(3): 188-190
DOI: 10.1055/s-2007-969982
Research Articles

© Hippokrates Verlag Stuttgart

Phytochemical Assessment of Lathyrus Species for the Neurotoxic Agent, β-N-oxalyl-L-α-β-Diaminopropionic Acid

C. J. Briggs1 , N. Parreno1 , C. G. Campbell2
  • 1Faculty of Pharmacy, University of Manitoba, Canada
  • 2Agriculture Canada Research Station, Morden, Manitoba, Canada
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Publication History

1982

Publication Date:
26 March 2007 (online)

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Abstract

Lathyrus species have potential as feed crops, but many contain a neurotoxin, β-N-oxalyl-L-α-β-diaminopropionic acid (ODAP). An extraction and Spectrophotometric assay procedure are described for analysis of ODAP in small samples (0.5 g) of seed. The assay utilizes o-phthalaldehyde as a reagent, following hydrolysis of the ODAP. It is sensitive and specific, and suitable for the assay of 3-4 seeds of most Lathyrus species. The method has been used to screen a collection of Lathyrus accessions for use in a breeding program to develop agronomic strains with low toxicity.

Key Word Index

Lathyrus - Fabaceae (Leguminosae) - β-N-oxalyl-L-α-β-Diaminopropionic Acid - Spectrophotometric Assay - Screening for Plant Breeding