Two high saponin-producing cell lines were selected from the original callus induced
in 1967 and maintained in good state. For saponin production and growth in two cell
lines, the comparison of static and suspension cultures, static cultures in the dark
or in the light and rotary or reciprocal shaking cultures were made in detail. It
was shown that the growth in rotary shaking cultures were 1.8 times higher than in
reciprocal cultures, while the saponin production was almost the same, and the most
effective hormonal condition was the combination of indole-3-butyric acid with kinetin.
The saponin content per fresh weight was almost the same as that of the crude drug,
whereas that per dry weight was 4 times higher.
Panax ginseng
- Araliaceae - Cell suspension culture - Cell lines - Ginseng saponins - Ginsenosides
Ra, Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2, Rh, Ro - HPLC
