Assay Methods for Antihepatotoxic Activity Using Peroxide-Induced Cytotoxicity in Primary Cultured Hepatocytes1

Yoshinobu Kiso; Osamu Kato; Hiroshi Hikino

doi:10.1055/s-2007-969391

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Planta Med 1985; 51(1): 50-52
DOI: 10.1055/s-2007-969391

Research Articles

Assay Methods for Antihepatotoxic Activity Using Peroxide-Induced Cytotoxicity in Primary Cultured Hepatocytes¹

Yoshinobu Kiso, Osamu Kato, Hiroshi Hikino

Pharmaceutical Institute, Tohoku University, Aoba-yama, Sendai, Japan

1 Liver-protective drugs, Part 26. Also Part 88 in the series on the validity of the Oriental medicines

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Publication History

1984

1984

Publication Date:
26 February 2007 (online)

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Abstract

Conditions were investigated to devise in vitro assay methods for antihepatotoxic activity using peroxide-induced damage in primary cultured rat liver cells. Utilizing linoleic acid peroxide, benzoyl peroxide, cumene hydroperoxide and adenosine diphosphate/Fe³⁺, satisfactory assay procedures were established. Some natural products known to exert liver-protective effects in vivo were screened to reveal that glycyrrhetinic acid, glycyrrhizin, cynarin, silybin and desoxypodophyllotoxin showed similar antihepatotoxic activity in these four assay methods.