Tropane Alkaloids from Atropa belladonna; Part I. Capillary Gas Chromatographic Analysis

Markku Ylinen; Toivo Naaranlahti; Seppo Lapinjoki; Aarre Huhtikangas; Maija-Liisa Salonen; Liisa Kaarina Simola; Mauri Lounasmaa

doi:10.1055/s-2007-969086

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Planta Med 1986; 52(2): 85-87
DOI: 10.1055/s-2007-969086

Full Papers

Tropane Alkaloids from Atropa belladonna; Part I. Capillary Gas Chromatographic Analysis

Markku Ylinen¹ , Toivo Naaranlahti¹ , Seppo Lapinjoki¹ , Aarre Huhtikangas¹ , Maija-Liisa Salonen² , Liisa Kaarina Simola² , Mauri Lounasmaa³

¹Department of Pharmaceutical Chemistry, University of Kuopio POB 6, SF-70211 Kuopio, Finland
²Department of Botany, University of Helsinki, Unioninkatu 44, SF-00170 Helsinki, Finland
³Department of Chemistry, Technical University of Helsinki, SF-02150 Espoo, Finland

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Publication History

1985

Publication Date:
26 February 2007 (online)

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Abstract

Atropine and scopolamine were assayed in leaf samples and cultured cells of Atropa belladonna L. by capillary gas chromatography. The samples were extracted and purified before the gas Chromatographic analysis by passage through disposable extraction columns of reversed phase type. Deviation among the analyses was 4.0% for atropine and 2.9% for scopolamine at 50 mg/l. The limit of detection was 5 mg/l for both alkaloids.