Int J Sports Med 1988; 09(4): 245-248
DOI: 10.1055/s-2007-1025014

© Georg Thieme Verlag Stuttgart · New York

Excretion of 3-Methylhistidine and Hydroxyproline Following Acute Weight-Training Exercise

C. A. Horswill, D. K. Layman, R. A. Boileau, B. T. Williams, B. H. Massey
  • Departments of Kinesiology and Nutritional Sciences, University of Illinois-Urbana-Champaign and Mercy Hospital Pathology Laboratory, Urbana, IL
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Publication History

Publication Date:
14 March 2008 (online)


Changes in urine excretion of 3-methylhistidine (3MH) and hydroxyproline (OHP) were investigated following a single session of weight training that produced delayed onset muscle soreness (DOMS). The subjects (n = 18) maintained meat-free diets for 6 days and collected 24-h urine volume on day 4 (PRE), day 5 (POST1), and day 6 (POST2) for analysis of 3MH, OHP, and creatinine (CRE). At the end of PRE, nine subjects (EX) performed intensive weight-training exercises. The remaining subjects (NE) served as a nonexercised group. All subjects were measured for fat free weight (FFW) and rated DOMS at the end of each urine collection period, i.e., PRE, POST1, and POST2. The results were significant DOMS ratings on POST1 and POST2 compared with PRE2 for EX only. Excretion of 3MH (x ± SE in µmol/day) by group were, for EX, PRE 271 ± 18, POST1 243 ± 16, POST2 259 + 23; for NE, PRE 203 ± 23, POST1 209 ± 27, POST2 225 ± 32. Excretion of OHP (x ± SE in mg/day) were, for EX, PRE 30.0 ± 5.4, POST1 32.8 ± 6.4, POST2 29.6 ± 6.1; for NE, PRE 22.5 ± 5.2, POST1 21.6 ± 6.0, POST2 22.7 ± 6.0. Changes in urine 3MH and OHP were not statistically significant, whether analyzed in absolute terms or relative to CRE and FFW. It was concluded that the acute session of weight training which produced muscle soreness did not effect excretion of 3MH or OHP.