Int J Sports Med 1992; 13(5): 436-438
DOI: 10.1055/s-2007-1021294
Short Communications

© Georg Thieme Verlag Stuttgart · New York

Altered Cellular Distribution of Hexokinase in Skeletal Muscle after Exercise

D. R. Van Houten, J. M. Davis, D. M. Meyers, J. L. Durstine
  • Department of Exercise Science, Exercise Biochemistry Laboratory, University of South Carolina, Columbia, South Carolina 29208
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Publication History

Publication Date:
14 March 2008 (online)


The purpose of this study was to determine the effects of a single period of swim exercise to exhaustion on the cellular distribution of hexokinase in rat skeletal muscle. The gastrocnemius muscle from male rats exercised (n = 16) to exhaustion or allowed to remain inactive (n = 14) was analyzed for hexokinase activity (HK) in the whole muscle homogenate and the mitochondrial-rich fraction. Although exercise produced a slight, non-significant, increase in HK activity in the whole muscle homogenate (p < 0.09), HK activity in the mitochondrial-rich fraction was significantly increased in the exercised muscle (p < 0.02). This shift in the cellular distribution of HK is towards the more active mitochondrial bound form and indicates an enhanced regulation of HK activity during physical exercise.