Immunoperoxidase staining was performed to localize glycoprotein hormone alpha subunit
in mouse brain. Staining was found only in the median eminence with the use of ovine
LH-alpha antiserum and was diffuse, not localized to fibers but to pericapillary areas.
Absorption of the antiserum with bovine TSH-alpha subunit completely abolished the
staining. These findings, in conjunction with our earlier studies in which alpha subunit
messenger RNA was not detected in mouse brain, suggest that alpha subunit reaches
the hypothalamus by retrograde diffusion from the pituitary.
Immunocytochemistry
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Alpha Subunit of Glycoprotein Hormones
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Mouse Brain
