Change of Skeletal Muscles Pyruvate Dehydrogenase Complex Activities in Congenital Diabetic Mice (KK Mice) by Aging or Contraction

Summary

The activity of pyruvate dehydrogenase complex was measured in skeletal muscle of congenital diabetic mice (KK mice) and control mice (ddN mice), each group in a starved or unstarved state, with or without muscular contraction.

The age related increment of the level of active form pyruvate dehydrogenase complex in KK mice was not found compared with that in ddN mice. In 4 weeks old KK mice, the increment of the active form by muscular contraction or the decrease by 48 hours starvation was not different from the control mice. On the other hand, in 12 week old KK mice, the changes of the enzyme activity by muscular contraction were different from the normal control.

These results suggest that the activity of pyruvate dehydrogenase complex is intimately related to the onset of diabetes.

Key-Words:

Pyruvate Dehydrogenase Complex - KK Mice - Skeletal Muscle - Muscular Contraction