Hormone and Metabolic Research, Table of Contents Horm Metab Res 1983; 15(7): 335-339DOI: 10.1055/s-2007-1018712 © Georg Thieme Verlag, Stuttgart · New York [Na+ + K+]ATP-ase in Liver and Brain of Obese Mice S. Hughes, D. A. York Department of Nutrition, University of Southampton, Southampton, England Recommend Article Abstract PDF Download Buy Article Summary The activity of hepatic [Na+ + K+]ATP-ase showed a genedosage relationship in 6 week old mice. Before weaning hepatic [Na+ + K+]ATP-ase activity was normal in preobese mice but fell within 7 days of weaning to the low levels observed in older ob/ob mice. Brain [Na+ + K+]ATP-ase activity was unchanged in ob/ob mice although [3H]-ouabain binding was reduced. Arrhenius plots of [Na+ + K+]ATP-ase activity in liver and brain and of [3H]-ouabain binding to brain preparations showed breakpoints at lower temperatures in ob/ob than lean mice. These breakpoints were altered by pretreatment of tissue with deoxycholate. It is suggested that changes in membrane lipid composition might be an important factor regulating [Na+ + K+]ATP-ase in ob/ob mice Key-Words: Gene-Dosage - Age - Arrhenius Plots - Deoxycholate PDF (498 kb)
