Horm Metab Res 1984; 16(2): 74-78
DOI: 10.1055/s-2007-1014701
© Georg Thieme Verlag, Stuttgart · New York

Inhibitory Effects of Somatostatin on Growth and Differentiation in Cultured Intestinal Mucosa

E. F. Stange, A. Schneider, V. Schusdziarra1 , H. Ditschuneit
  • Zentrum für Innere Medizin der Universitát Ulm, Abteilung für Innere Medizin II, Ulm, Germany
  • 1Abteilung für Innere Medizin I, Ulm, Germany
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Publikationsverlauf

1982

1983

Publikationsdatum:
14. März 2008 (online)

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Summary

The effect of somatostatin on mucosal DNA, protein and brush border enzymes was studied in organ cultured rabbit jejunum and ileum.

Compared to control cultures, somatostatin reduced the biopsy DNA and protein content in parallel in the jejunum, but was ineffective in the ileum. This was probably due to a direct growth inhibition, since DNA and brush border enzyme activity from desquamated cells in the postculture medium were unaffected.

In addition, a direct inhibition of jejunal villous cell differentiation by somatostatin was reflected in a significant decrease of sucrase, maltase and alkaline phosphatase activity. In the ileum, only the specific activity of alkaline phosphatase was reduced. The key enzyme of cholesterol synthesis, HMG-CoA-reductase, was measured as an intracellular enzyme control and was not influenced by the hormone.

The high somatostatin concentrations necessary to achieve the effects are not an artefact of hormone degradation during culture, as shown by radioimmunoassay, and suggest a local or “paracrine” rather than systemic, inhibitory action of somatostatin on intestinal growth and differentiation.