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Horm Metab Res 1985; 17(10): 541-543
DOI: 10.1055/s-2007-1013599
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© Georg Thieme Verlag, Stuttgart · New York

Islet Transplantation in Experimental Diabetes of the Rat

X. Induction of Cytotoxic Antibodies and Islet-Cell-Surface-Antibodies after Homologous Islet Transplantation in RatsT. Zekorn, R. Bretzel, K. Federlin
  • III. Med. Klinik u. Poliklinik d. Universität Gießen, Gießen, Germany
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Publication History

1985

1985

Publication Date:
14 March 2008 (online)

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Summary

The mechanisms of islet allograft rejection are still unknown. 350 allogeneic rat islets (BDE/Han) were transplanted into the liver of Streptozotocin-diabetic rats (Lewis/Han). Animals were killed either on day 4, 7, 10 or 14.

Immunfluorescence analysis for islet-cell-surface-antibodies (ICSA) was performed on BDE-islet-single-cells yielded by Collagenase-Dispase digestion. In order to detect cytoplasmic islet cell antibodies, immunfluorescence technique was used on BDE-pancreas cryosections. Cytotoxicity assay was tried out using 51Cr-release-test.

After successful intraportal transplantation, rejection took place between day 7 and 10. ICSA could be observed on day 10 and 14. Cytoplasmic antibodies were not detected at any time. A specific cytotoxic activity in the recipient serum revealed itself on day 10 (23.0%) and day 14 (27.7%).

Transplantation of freshly isolated allogeneic islets induced ICSA and cytotoxic antibodies. These results indicate an involvement of humoral mechanisms in islet allograft rejection.

Key-Words:

Allogeneic Islet Transplantation - Rats - Islet-Cell-Surface-Antibodies - Cytoplasmic Antibodies - Cytotoxicity Assay