Insulin Stimulates Glucose Transport in Isolated Human Adipose Cells Through a Translocation of Intracellular Glucose Transporters to the Plasma Membrane: A Preliminary Report

E. Karnieli; B. Chernow; P. J. Hissin; I. A. Simpson; J. E. Foley

doi:10.1055/s-2007-1012458

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 1986; 18(12): 867-868
DOI: 10.1055/s-2007-1012458

SHORT COMMUNICATIONS

Insulin Stimulates Glucose Transport in Isolated Human Adipose Cells Through a Translocation of Intracellular Glucose Transporters to the Plasma Membrane: A Preliminary Report

E. Karnieli¹ , B. Chernow² , P. J. Hissin, I. A. Simpson, J. E. Foley

¹Metabolic Unit, Rambam Medical Center, Faculty of Medicine Technicon, Haifa, Israel;
²Department of Surgery, Bethesda Naval Hospital, Bethesda, Maryland;
Experimental Diabetes, Metabolism and Nutrition Section, Molecular, Cellular and Nutritional Endocrinology Branch, and National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland;
Clinical Diabetes and Nutrition Section, Phoenix Epidemiology and Clinical Research Branch National Institute of Diabetes, and Digestive and Kidney Diseases, National Institutes of Health, Phoenix, Arizona, U.S.A.

Abstract

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Summary

Insulin's effects on glucose transport activity and the subcellular distribution of glucose transporters have been examined in isolated human abdominal adipose cells, by measuring 3-0-methylgulcose transport and specific D-glucose-inhibitable cytochalasin B binding to plasma membranes and low-density microsomes, respectively. Insulin appears to stimulate glucose transport in isolated human adipose cell through the translocation of glucose transporters from a large intracellular pool to the plasma membrane as initially postulated for rat adipose and muscle cells.

Key-Words

Insulin - Glucose Transport - Glucose Transporter - Human Adipose Cells

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