Summary
Sixty-two children, aged 2-18 years, suspected of an endocrine disorder were given
one of several IM injections of the LHRH and the blood was drawn prior to and at 30,
60, 90, 120, 240 minutes after the injection. An aliquot of .2 ml of serum was taken
from each post-LHRH specimen and pooled. The gonadotropins were measured in all the
individual and pooled samples. A high correlation (r=.974, LH; r=.981, FSH) between
the peak and the pooled sample suggests that the analysis of gonadotropins in a pooled
sample gives information comparable to that obtained by the gonadotropin analysis
in multiple serum samples. A formula to calculate the peak LH and FSH from the pooled
specimen taken from only four post-LHRH injections is: peak LH = 3.015 + 1.049 times
the pooled LH; peak FSH = 3.153 + 1.072 times the pooled FSH value. The correlation
coefficient between the observed and the calculated LH was .98; the same for the FSH
was .987. I suggest that the pooled sample, due to it's integrated response, is a
better reflection of the pituitary response to the LHRH.
Key-Words
LHRH
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Gonadotropin
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Diagnostic Use
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Children
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Method
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Pooled Sample
