Summary
Immunoreactive arginine vasopressin (irAVP) was measured in seminal fluid with and
without extraction using a specific radioimmunoassay (RIA). A large fraction of irAVP
was removed after extraction on octadecasilylsilica cartridges. The measured amount
of irAVP corresponded to the levels found in blood plasma. Dilutions of seminal plasma
extracts were parallel with the RIA standard curve. On reversed phase HPLC the extracted
material coeluted with synthetic AVP. These findings suggest an identity of this immunoreactive
material with intact AVP. During incubations of synthetic AVP and its analogue 8-D-arginine
vasopressing (8-DAVP) in seminal plasma, immunoreactivity decreased considerably with
the former peptide, while the concentration of 8-DAVP was not significantly altered.
Key-Words
Arginine Vasopressin
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Radioimmunoassay
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Sperm
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Cattle
