A Sensitive Radioimmunoassay for Somatomedin-C/Insulin-Like Growth-Factor I Based on Synthetic Insulin-Like Growth Factor 57-70

R. L. Hintz; F. Liu; D. Chang; G. Seegan

doi:10.1055/s-2007-1010832

Hormone and Metabolic Research, Inhaltsverzeichnis

Horm Metab Res 1988; 20(6): 344-347
DOI: 10.1055/s-2007-1010832

Clinical

A Sensitive Radioimmunoassay for Somatomedin-C/Insulin-Like Growth-Factor I Based on Synthetic Insulin-Like Growth Factor 57-70

R. L. Hintz, F. Liu, D. Chang, G. Seegan

The Department of Pediatrics, Stanford University, Stanford, U.S.A.
Peninsula Laboratories, Belmont, U.S.A.
Endocrine Science Laboratories, Tarzana, U.S.A.

Abstract

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Summary

We have developed a region-specific radioimmunoassay for somatomedin-C/insulin-like growth factor-I (IGF-I) directed against IGF-I 57-70. This sequence includes part of the A region and all of the carboxyterminal D region. In addition to being highly specific, this immunoassay is sensitive to as little as 1 ng/ml of IGF-I, and has half-maximum displacement by 10 ng/ml of IGF-I. Comparison to other existent immunoassays for IGF-I shows correlation coefficients of > 0.95. This immunoassay has the advantage of being directed against a known region of the IGF-I molecule which is on the exterior of the 3-dimensional structure, and can use a readily available synthetic peptide as both radioligand and standard.

Key-Words

Somatomedin - Insulin-like Growth Factor - Radioimmunoassay

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