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Horm Metab Res 1989; 21(11): 619-622
DOI: 10.1055/s-2007-1009302
Clinical

© Georg Thieme Verlag, Stuttgart · New York

Measurement of Proinsulin in Human Pancreatic Juice

S. Murozono1 , K. Sato2 , J. Picazo3 , H. Ishii4
  • 1Meikai University, Faculty of Economics, Department of Economics, Chemical Laboratory, Chiba, Japan
  • 2Meikai University, School of Dentistry, Radioisotope Laboratory, Saitama, Japan
  • 3Novo Research Institute, Bagsvard, Denmark
  • 4Asahigaoka Hospital, Saitama, Japan
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Publication History

1988

1989

Publication Date:
14 March 2008 (online)

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Summary

In this study, we measured proinsulin in human pancreatic juice by radioimmunoassay (RIA). Affinity chromatography was used to separate proinsulin and insulin from C-peptide; and high-performance liquid chromatography, to separate proinsulin from insulin. In the RIA procedure for proinsulin we used porcine insulin antiserum as the antibody and porcine proinsulin as the standard and labelled antigen.

The concentrations of proinsulin in human pancreatic juice obtained 5, 10, 15 and 20 minutes after intravenous injection of secretin were 29 ± 6, 41 ± 4, 35 ± 14 and 37 ± 17 (pmol/l ± SD, means of 7 subjects), respectively. These values were higher than those in serum from fasted subjects, 12 ± 4 pmol ± SD, This work shows that proinsulin is present in human pancreatic juice.

Key-Words

Human Pancreatic Juice - Proinsulin - HPLC - Affinity Chromatography - RIA