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Horm Metab Res 1990; 22(4): 229-231
DOI: 10.1055/s-2007-1004890
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© Georg Thieme Verlag, Stuttgart · New York

Electrofocusing Analysis of DNA Polymerase β in Hypophysectomized Rat Testis

A. Nagasaki1 , S. Yoshida2 , Takako Ohyama1 , Rumi Masunaga1 , S. Kato1 , Mika Suzuki1 , Motoko Itoh1 , T. Kawabe1
  • 1Department of Internal Medicine, Fujita Health University School of Medicine, Toyoake, Aichi
  • 2Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Showa-ku, Nagoya, Japan
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Publication History

1988

1989

Publication Date:
14 March 2008 (online)

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Summary

Isoelectric focusing studies indicated that three peaks of DNA polymerase β (β-enzyme) activity were focused at isoelectric point of 7.1, 8.2 and 9.1 in testes of sham-operated rats, whereas in testes of hypophysectomized rats only two peaks of the β-enzyme activity appeared at pH 7.1 and 9.1 and the peak at pH 8.2 was reconstituted by the treatment of gonadotropins and testosterone. In the brain hypophysectomy did not affect the activities of isoelectrically different β-enzymes.

These facts imply that under in vivo conditions the β-enzymes in testes focused at different isoelectric points are modified with unknown factors and the β-enzyme focused at pH 8.2 is affected by pituitary hormones.

Key words

Electrofocusing - DNA Polymerase - Hypophysectomized Rat Testis