Effects of Continuous Low-carbohydrate Diet after Long-term Exercise on GLUT-4 Protein Content in Rat Skeletal Muscle

M. Kubota; K. Koshinaka; Y. Kawata; T. Koike; Y. Oshida

doi:10.1055/s-2007-1004530

Hormone and Metabolic Research, Inhaltsverzeichnis

Horm Metab Res 2008; 40(1): 24-28
DOI: 10.1055/s-2007-1004530

Original Basic

Effects of Continuous Low-carbohydrate Diet after Long-term Exercise on GLUT-4 Protein Content in Rat Skeletal Muscle

M. Kubota¹ , K. Koshinaka² , Y. Kawata³ , T. Koike³ ^, ⁴ , Y. Oshida³ ^, ⁴

¹Department of Health Science, Faculty of Medicine, Kyoto University, Kyoto, Japan
²Department of Health and Nutrition, Faculty of Medical Technology, Niigata University of Health and Welfare, Niigata, Japan
³Department of Sports Medicine, Graduate School of Medicine, Nagoya University, Nagoya, Japan
⁴Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya, Japan

Abstract

Stimulation of AMPK and decreased glycogen levels in skeletal muscle have a deep involvement in enhanced insulin action and GLUT-4 protein content after exercise training. The present study examined the chronic effects of a continuous low-carbohydrate diet after long-term exercise on GLUT-4 protein content, glycogen content, AMPK, and insulin signaling in skeletal muscle. Rats were divided randomly into four groups: normal chow diet sedentary (N-Sed), low carbohydrate diet sedentary (L-Sed), normal chow diet exercise (N-Ex), and low carbohydrate diet exercise (L-Ex) groups. Rats in the exercise groups (N-Ex and L-Ex) were exercised by swimming for 6 hours/day in two 3-hour bouts separated by 45 minutes of rest. The 10-day exercise training resulted in a significant increase in the GLUT-4 protein content (p<0.01). Additionally, the GLUT-4 protein content in L-Ex rats was increased by 29% above that in N-Ex rats (p<0.01). Finally, the glycogen content in skeletal muscle of L-Ex rats was decreased compared with that of N-Ex rats. Taken together, we suggest that the maintenance of glycogen depletion after exercise by continuous low carbohydrate diet results in the increment of the GLUT-4 protein content in skeletal muscle.

Key words

glucose - glycogen - epitrochlearis - training - AMPK

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Correspondence

Y. OshidaMD, PhD

Department of Sports Medicine

Graduate School of Medicine

Nagoya University

Nagoya 464-8601

Japan

Telefon: +81/52/789 39 61

Fax: +81/52/789 39 57

eMail: oshida@htc.nagoya-u.ac.jp