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Horm Metab Res 1991; 23(11): 522-529
DOI: 10.1055/s-2007-1003746
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© Georg Thieme Verlag, Stuttgart · New York

Effect of Castration and Anatomical Site on the Plasma Membrane Structure and the Activities of Adenylate Cyclase and 5'-Nucleotidase in Pig Adipocytes

Christine Nicolas, Y. Demarne, Marie-José Lecourtier, Claude Lhuillery
  • Laboratoire de Nutrition et Sécurité Alimentaire, INRA-CRJ, Domaine de Vilvert, Jouy-en-Josas, France
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Publication History

1990

1991

Publication Date:
14 March 2008 (online)

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Summary

The effects of castration on fat cell plasma membrane structure and enzyme activities (adenylate cyclase and 5'-nucleotidase) were studied in pig adipose tissues in two fat deposits (subcutaneous and perirenal). Castration induced a fat cell enlargement in both tissues. Membrane cholesterol content was reduced and fluidity was increased in perirenal fat from castrated animals. Castration had no effect on 5'-nucleotidase activity which was higher in subcutaneous than in perirenal in both kinds of animals. Adenylate cyclase activity was studied in the presence of different effectors: isoproterenol-stimulations of the enzyme were not affected by castration but were site-specific. GppNHp-stimulated activities were increased in subcutaneous fat from castrated animals. Castration had no influence on forskolin stimulations. The magnitude of GppNHp- and forskolin-stimulated activities were found to be tissue-dependent.

Membrane results are discussed in relation with castration-induced fat cell enlargement.

Key words

Pig Adipocytes - Adenylate Cyclase - 5'-Nucleotidase - Membrane Fluidity - Gonadal Steroids - Fat Distribution

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