Amylin is a normal secretory protein of the pancreatic β-cells as well as a major
constituent of the islet amyloid deposits in patients with non-insulin-dependent diabetes
mellitus. We studied the effects of amylin on the release of insulin and glucagon
from the isolated perfused rat pancreas. Rat amylin was dissolved in basal perfusates
to a final concentration of 100 nM. Amylin did not alter glucose-stimulated secretion
of insulin but significantly inhibited arginine-stimulated secretion of insulin (control:
20.9±1.4 pmol/min; amylin group: 14.8±1.6 pmol/min, p<0.05). Amylin did not alter
the release of glucagon from the perfused rat pancreas in response to 16.7 mM glucose
and 10 mM arginine. These findings suggest that amylin may modulate the secretion
of insulin from pancreatic β-cells.
Islet Amyloid Polypeptide - Amylin - Insulin - Glucagon - Pancreas Perfusion
