Fluctuations of Carbohydrates in Haemolymph of Honeybee (Apis mellifica) After Fasting, Feeding and Stress

M. A. M. Abou-Seif; V. Maier; J. Fuchs; M. Mezger; E. F. Pfeiffer; M. Bounias

doi:10.1055/s-2007-1002034

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Horm Metab Res 1993; 25(1): 4-8
DOI: 10.1055/s-2007-1002034

Originals Basic

Fluctuations of Carbohydrates in Haemolymph of Honeybee (Apis mellifica) After Fasting, Feeding and Stress

M. A. M. Abou-Seif¹ , V. Maier¹ , J. Fuchs¹ , M. Mezger¹ , E. F. Pfeiffer¹ , M. Bounias²

¹Abteilung Innere Medizin I der Universität Ulm, Ulm, Germany
²Laboratoire de Biochimie, INRA, Centre de Recherches d'Avignon, Montfavet, France

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Publikationsverlauf

1991

1991

Publikationsdatum:
14. März 2008 (online)

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Summary

The haemolymph of honeybee contains trehalose, a non-reducing disaccharide, glucose and fructose. The aim of this work was to describe changes of haemolymph sugar induced by different physiological situations. Gas chromatography for the determination of carbohydrate concentrations in the haemolymph was introduced. The individual bee was anesthetized by CO₂ and punctured at tergum III in order to gain 1 μl haemolymph using a glass capilette. The main sugars in the haemolymph were determined as silylated derivatives by gas chromatography. Bees living under exactly standardized conditions in an artificial bee-house were observed after feeding and fasting. In addition saccharides were determined after “run-stress” along different distances.

Key words

Honeybee (Apis mellifica) - Haemolymph Carbohydrates - Gas Chromatography of Carbohydrates