A Method for the Quantitative Determination of Anthraquinones and Alkaloids in Cell and Tissue Cultures of Cinchona Sp.

R. Wijnsma; T. B. van Vliet; P. A. A. Harkes; H. J. van Groningen; R. van der Heijden; R. Verpoorte; A. Baerheim Svendsen

doi:10.1055/s-2006-962627

Planta Med 1987; 53(1): 80-84
DOI: 10.1055/s-2006-962627

Full Papers

A Method for the Quantitative Determination of Anthraquinones and Alkaloids in Cell and Tissue Cultures of Cinchona Sp.

R. Wijnsma¹ , T. B. van Vliet² , P. A. A. Harkes² , H. J. van Groningen¹ , R. van der Heijden¹ , R. Verpoorte¹ , A. Baerheim Svendsen¹

¹Biotechnology Delft Leiden (BDL), Section of Pharmacognosy, Center for Bio-Pharmaceutical Sciences, Leiden University, Gorlaeus Laboratories, P. O. Box 9502, 2300 RA Leiden, The Netherlands.
²Biotechnology Delft Leiden (BDL), Department of Plant Molecular Biology, Subfaculty of Biology, Leiden University, Nonnensteeg 3, 2311 VJ Leiden, The Netherlands.

Abstract

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Abstract

A procedure is described for the extraction of both anthraquinones and alkaloids from tissue culture material of Cinchona species. Using this extraction method, it is possible to quantitatively determine the two groups of secondary metabolites produced in Cinchona tissue cultures.

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