Abstract
Isobyakangelicin angelate, a new furanocoumarin and the five known coumarins, columbianadin,
(+)-oxypeucedanin, (±)-oxypeucedanin hydrate, ostruthol, and isoimperatorin, were
isolated from roots of Peucedanum palustre (L.) Moench using medium-pressure liquid chromatography [MPLC] with normal phase
and subsequently with reversed phase as stationary phases. The mobile phases were
optimized in TLC and HPLC preassays according to the “PRISMA” system and then transferred
without modification to preparative MPLC separations. The structures of the compounds
were elucidated by a combination of spectroscopic methods, among others the rotating
frame Overhauser enhancement spectroscopy [ROESY] experiment.
