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DOI: 10.1055/s-2006-960990
© Georg Thieme Verlag Stuttgart · New York
Fractionation and Characterization of Mitogenic and Anti-Complementary Active Fractions from Kampo (Japanese Herbal) Medicine “Juzen-Taiho-To”1
1 Studies on Immunologically Active Substances from Kampo Medicine “Juzen-Taiho-To”, Part 1Publikationsverlauf
1989
Publikationsdatum:
05. Januar 2007 (online)
Abstract
“Juzen-Taiho-To” (TJ-48), which is a kampo (Japanese herbal) medicine prepared by decocting a prescription of ten kinds of herbs, has several immunostimulating activities. In order to characterize the active substances for anti-complementary and mitogenic activities, TJ-48 was fractionated. Anti-complementary activity was observed in the water- and methanol-insoluble fraction (F-2) and the crude polysaccharide fraction (F-5), whereas mitogenic activity was only found in F-5. However, other low molecular mass fractions did not show both activities. Methylation analysis indicated that F-2 mainly contained amylopectin-like polysaccharides. Both Pronase digestion and periodate oxidation decreased the anti-complementary activity of F-2, and the β-amylase-resistant fraction of F-2 still retained the potent anti-complementary activity.
When F-5, which has the most potent of both activities, was further fractionated, only the major acidic polysaccharide fraction, F-5-2, showed potent mitogenic activity. F-5-2 was also shown to have the highest anti-complementary activity. Endo-α-(1→4)-polygalacturonase digestion showed that F-5-2 mainly contained pectic polysaccharides, and the endo-polygalacturonase treatment of F-5-2 reduced the mitogenic activity but not the anti-complementary activity. F-2 and F-5 each activated the complement system by a different mode of action.
Key words
Anti-complementary activity - pectic polysaccharide - kampo (Japanese herbal) medicine - mitogenic activity